{\rtf1\adeflang1025\ansi\ansicpg936\uc2\adeff0\deff0\stshfdbch13\stshfloch0\stshfhich0\stshfbi0\deflang1033\deflangfe2052{\fonttbl{\f0\froman\fcharset0\fprq2{\*\panose 02020603050405020304}Times New Roman;}{\f1\fswiss\fcharset0\fprq2{\*\panose 020b0604020202020204}Arial{\*\falt Arial};} {\f13\fnil\fcharset134\fprq2{\*\panose 02010600030101010101}\'cb\'ce\'cc\'e5{\*\falt ..\'a8\'ac.};}{\f36\fnil\fcharset134\fprq2{\*\panose 02010600030101010101}@\'cb\'ce\'cc\'e5;}{\f39\froman\fcharset238\fprq2 Times New Roman CE;} {\f40\froman\fcharset204\fprq2 Times New Roman Cyr;}{\f42\froman\fcharset161\fprq2 Times New Roman Greek;}{\f43\froman\fcharset162\fprq2 Times New Roman Tur;}{\f44\fbidi \froman\fcharset177\fprq2 Times New Roman (Hebrew);} {\f45\fbidi \froman\fcharset178\fprq2 Times New Roman (Arabic);}{\f46\froman\fcharset186\fprq2 Times New Roman Baltic;}{\f47\froman\fcharset163\fprq2 Times New Roman (Vietnamese);}{\f49\fswiss\fcharset238\fprq2 Arial CE{\*\falt Arial};} {\f50\fswiss\fcharset204\fprq2 Arial Cyr{\*\falt Arial};}{\f52\fswiss\fcharset161\fprq2 Arial Greek{\*\falt Arial};}{\f53\fswiss\fcharset162\fprq2 Arial Tur{\*\falt Arial};}{\f54\fbidi \fswiss\fcharset177\fprq2 Arial (Hebrew){\*\falt Arial};} {\f55\fbidi \fswiss\fcharset178\fprq2 Arial (Arabic){\*\falt Arial};}{\f56\fswiss\fcharset186\fprq2 Arial Baltic{\*\falt Arial};}{\f57\fswiss\fcharset163\fprq2 Arial (Vietnamese){\*\falt Arial};} {\f171\fnil\fcharset0\fprq2 SimSun Western{\*\falt ..\'a8\'ac.};}{\f401\fnil\fcharset0\fprq2 @\'cb\'ce\'cc\'e5 Western;}}{\colortbl;\red0\green0\blue0;\red0\green0\blue255;\red0\green255\blue255;\red0\green255\blue0;\red255\green0\blue255; \red255\green0\blue0;\red255\green255\blue0;\red255\green255\blue255;\red0\green0\blue128;\red0\green128\blue128;\red0\green128\blue0;\red128\green0\blue128;\red128\green0\blue0;\red128\green128\blue0;\red128\green128\blue128;\red192\green192\blue192; \red255\green255\blue255;}{\stylesheet{\qj \li0\ri0\nowidctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\f0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 \snext0 \styrsid15547592 Normal;}{\*\cs10 \additive \ssemihidden Default Paragraph Font;}{\* \ts11\tsrowd\trftsWidthB3\trpaddl108\trpaddr108\trpaddfl3\trpaddft3\trpaddfb3\trpaddfr3\tblind0\tblindtype3\tscellwidthfts0\tsvertalt\tsbrdrt\tsbrdrl\tsbrdrb\tsbrdrr\tsbrdrdgl\tsbrdrdgr\tsbrdrh\tsbrdrv \ql \li0\ri0\widctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs20 \ltrch\fcs0 \fs20\lang1024\langfe1024\loch\f0\hich\af0\dbch\af13\cgrid\langnp1024\langfenp1024 \snext11 \ssemihidden Normal Table;}{ \s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \snext15 Default;}{ \s16\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM1;}{ \s17\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM13;}{ \s18\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM3;}{\s19\ql \li0\ri0\sl551\slmult0 \nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM4;}{ \s20\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM15;}{\s21\ql \li0\ri0\sl540\slmult0 \nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM5;}{ \s22\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM16;}{ \s23\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM14;}{\s24\ql \li0\ri0\sl400\slmult0 \nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM10;}{\s25\ql \li0\ri0\sl631\slmult0 \nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\f1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 \sbasedon15 \snext15 CM12;}{\*\cs26 \additive \rtlch\fcs1 \af0 \ltrch\fcs0 \ul\cf2 \sbasedon10 \styrsid15547592 Hyperlink;}}{\*\latentstyles\lsdstimax156\lsdlockeddef0}{\*\listtable{\list\listtemplateid-1528444278\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0 {\leveltext\'01\'00;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid-1257957970}{\list\listtemplateid149623051\listhybrid{\listlevel\levelnfc23 \levelnfcn23\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext\'01?;}{\levelnumbers;}}{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;} \rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0 \levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;} \rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0 \levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;} \rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid-980556856} {\list\listtemplateid362574038\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext\leveltemplateid1081490614\'01\'00;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \loch\af0\hich\af0\dbch\af0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0 \levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;} \rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0 \levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;} \rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0 \levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid-296305642}{\list\listtemplateid296678860\listhybrid{\listlevel\levelnfc23\levelnfcn23\leveljc0\leveljcn0\levelfollow0 \levelstartat1\levelspace0\levelindent0{\leveltext\'01?;}{\levelnumbers;}}{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid391955264}{\list\listtemplateid141617770\listhybrid{\listlevel\levelnfc0 \levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext\'01\'00;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel \levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0 \levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid750042560}{\list\listtemplateid-638015300\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext\leveltemplateid-1595087598\'01\'00;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \loch\af0\hich\af0\dbch\af0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0 \leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext \'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0 \leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext \'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0 \leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid826056733}{\list\listtemplateid2082651688\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0 \leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext\leveltemplateid-2027762758\'01\'00;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 \loch\af0\hich\af0\dbch\af0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid1288321831}{\list\listtemplateid1274048997\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1\levelspace0\levelindent0{\leveltext\'02\'00.;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid1676094530}{\list\listtemplateid-60180834\listhybrid{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat1 \levelspace0\levelindent0{\leveltext\'01\'00;}{\levelnumbers\'01;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0 \levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listlevel\levelnfc0\levelnfcn0\leveljc0\leveljcn0\levelfollow0\levelstartat0\levelspace0\levelindent0{\leveltext\'00;}{\levelnumbers;}\rtlch\fcs1 \af0 \ltrch\fcs0 }{\listname ;}\listid1867824793}}{\*\listoverridetable {\listoverride\listid-980556856\listoverridecount0\ls1}{\listoverride\listid1676094530\listoverridecount0\ls2}{\listoverride\listid1867824793\listoverridecount0\ls3}{\listoverride\listid-296305642\listoverridecount0\ls4}{\listoverride\listid391955264 \listoverridecount0\ls5}{\listoverride\listid-1257957970\listoverridecount0\ls6}{\listoverride\listid750042560\listoverridecount0\ls7}{\listoverride\listid826056733\listoverridecount0\ls8}{\listoverride\listid1288321831\listoverridecount0\ls9}} {\*\rsidtbl \rsid922239\rsid1590987\rsid4222265\rsid9585049\rsid12795595\rsid13697845\rsid15547592\rsid16057590}{\*\generator Microsoft Word 11.0.0000;}{\info{\title Rat Insulin-like Growth Factor I ELISA kit}{\author user}{\operator user} {\creatim\yr2011\mo10\dy28\hr7\min48}{\revtim\yr2012\mo2\dy7\hr11\min40}{\version7}{\edmins1}{\nofpages11}{\nofwords1479}{\nofchars8436}{\nofcharsws9896}{\vern24615}{\*\password 00000000}}{\*\xmlnstbl {\xmlns1 http://schemas.microsoft.com/office/word/2003 /wordml}{\xmlns2 urn:schemas-microsoft-com:office:smarttags}}\paperw10831\paperh15015\margl1222\margr413\margt1733\margb564\gutter0\ltrsect \ftnbj\aenddoc\donotembedsysfont0\donotembedlingdata1\grfdocevents0\validatexml0\showplaceholdtext0\ignoremixedcontent0\saveinvalidxml0\showxmlerrors0\horzdoc\dghspace120\dgvspace120\dghorigin1701\dgvorigin1984\dghshow0\dgvshow3 \jcompress\viewkind1\viewscale120\rsidroot9585049 \fet0{\*\wgrffmtfilter 013f}\ilfomacatclnup0\ltrpar \sectd \ltrsect\linex0\sectdefaultcl\sftnbj {\*\pnseclvl1\pnucrm\pnstart1\pnindent720\pnhang {\pntxta \dbch .}}{\*\pnseclvl2 \pnucltr\pnstart1\pnindent720\pnhang {\pntxta \dbch .}}{\*\pnseclvl3\pndec\pnstart1\pnindent720\pnhang {\pntxta \dbch .}}{\*\pnseclvl4\pnlcltr\pnstart1\pnindent720\pnhang {\pntxta \dbch )}}{\*\pnseclvl5\pndec\pnstart1\pnindent720\pnhang {\pntxtb \dbch (} {\pntxta \dbch )}}{\*\pnseclvl6\pnlcltr\pnstart1\pnindent720\pnhang {\pntxtb \dbch (}{\pntxta \dbch )}}{\*\pnseclvl7\pnlcrm\pnstart1\pnindent720\pnhang {\pntxtb \dbch (}{\pntxta \dbch )}}{\*\pnseclvl8\pnlcltr\pnstart1\pnindent720\pnhang {\pntxtb \dbch (} {\pntxta \dbch )}}{\*\pnseclvl9\pnlcrm\pnstart1\pnindent720\pnhang {\pntxtb \dbch (}{\pntxta \dbch )}}\pard\plain \ltrpar\s15\qc \li0\ri0\sa2695\sl626\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1 \ltrch\fcs0 \insrsid15547592 \hich\af1\dbch\af13\loch\f1 }{\rtlch\fcs1 \ab\af1\afs52 \ltrch\fcs0 \b\fs52\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Human High Mobility Group protein B1(HMGB-1)ELISA Kit }{\rtlch\fcs1 \af1\afs52 \ltrch\fcs0 \fs52\insrsid15547592 \par }\pard\plain \ltrpar\s17\qc \li0\ri0\sa252\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Catalog No. CSB-E08223h }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \par }\pard\plain \ltrpar\s16\qc \li0\ri0\sa2350\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 (96 T) \par }\pard\plain \ltrpar\s15\ql \fi735\li0\ri0\sa146\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0\cufi350\pararsid922239 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 This immunoassay kit allows for the in vitro quantitative determination of }{\rtlch\fcs1 \ab\af1\afs21 \ltrch\fcs0 \b\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 human HMGB-1 }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 concentrations in }{\rtlch\fcs1 \ab\af1\afs21 \ltrch\fcs0 \b\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 serum,\hich\af1\dbch\af13\loch\f1 plasma. }{ \rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard \ltrpar\s15\ql \fi736\li0\ri0\sa146\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0\cufi350\pararsid922239 {\rtlch\fcs1 \ab\af1\afs21 \ltrch\fcs0 \b\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Expiration date six }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 months from the date of manufacture \par }\pard \ltrpar\s15\ql \fi736\li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0\cufi350\pararsid922239 {\rtlch\fcs1 \ab\af1\afs21 \ltrch\fcs0 \b\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard\plain \ltrpar\qj \li0\ri0\nowidctlpar\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0\itap0\pararsid15547592 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'cf\'c3\'c3\'c5\'bb\'db\'bc\'ce\'c9\'fa\'ce\'ef\'b3\'a4\'c6\'da\'be\'ad\'d3\'aa}{ \rtlch\fcs1 \af0 \ltrch\fcs0 \b\insrsid15547592\charrsid6121552 \hich\af0\dbch\af13\loch\f0 ELISA}{\rtlch\fcs1 \af0 \ltrch\fcs0 \b\insrsid15547592\charrsid6121552 \loch\af0\hich\af0\dbch\f13 \'ca\'d4\'bc\'c1\'ba\'d0}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'bc\'b0}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 Santa/Abcam}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'bf\'b9\'cc\'e5\'a1\'a2}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 Prospec}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'cf\'b8\'b0\'fb\'d2\'f2\'d7\'d3\'a1\'a2}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 Sigma/Amresco/Qiagen}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'a1\'a2}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 Axygen}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'ba\'c4\'b2\'c4\'b5\'c8\'c9\'fa\'ce\'ef\'ca\'d4\'bc\'c1\'b2\'fa\'c6\'b7\'a1\'a3\'b3\'cf\'d0\'c5\'be\'ad\'d3\'aa\'a3\'ac\'bc\'db\'b8\'f1\'ca\'b5\'bb\'dd\'a3\'ac\'b7\'fe\'ce\'f1\'d6\'dc\'b5\'bd\'a3\'ac\'d6\'ca \'c1\'bf\'d3\'d0\'b1\'a3\'d6\'a4\'a1\'a3\'bb\'b6\'d3\'ad{\*\xmlopen\xmlns2{\factoidname PersonName}{\xmlattr\xmlattrns0{\xmlattrname ProductID}{\xmlattrvalue \'b9\'e3\'b8\'e6}}}\loch\af0\hich\af0\dbch\f13 \'b9\'e3\'b8\'e6{\*\xmlclose}\'c0\'cf\'ca\'a6 \'c0\'b4\'d1\'af\'a3\'a1\'d7\'c9\'d1\'af\'b5\'e7\'bb\'b0}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 :18906011628 0592-6020891 QQ:}{\rtlch\fcs1 \af0 \ltrch\fcs0 \cf5\insrsid15547592\charrsid8725940 \hich\af0\dbch\af13\loch\f0 }{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592\charrsid8725940 \hich\af0\dbch\af13\loch\f0 1193952234 }{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 1048735792 }{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'bb\'f2\'b5\'c7\loch\af0\hich\af0\dbch\f13 \'c2\'bd}{\field\flddirty{\*\fldinst {\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 HYPERLINK "}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592\charrsid15887580 \hich\af0\dbch\af13\loch\f0 http://www.biohj.com/download.aspx}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \hich\af0\dbch\af13\loch\f0 " }{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 {\*\datafield 00d0c9ea79f9bace118c8200aa004ba90b02000000170000002300000068007400740070003a002f002f007700770077002e00620069006f0068006a002e0063006f006d002f0064006f0077006e006c006f00610064002e0061007300700078000000e0c9ea79f9bace118c8200aa004ba90b5e0000006800740074007000 3a002f002f007700770077002e00620069006f0068006a002e0063006f006d002f0064006f0077006e006c006f00610064002e0061007300700078000000795881f43b1d7f48af2c825dc485276300000000a5ab000000}}}{\fldrslt {\rtlch\fcs1 \af0 \ltrch\fcs0 \cs26\ul\cf2\insrsid15547592\charrsid925268 \hich\af0\dbch\af13\loch\f0 http://www.biohj.com/download.aspx}}}\sectd \linex0\sectdefaultcl\sftnbj {\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592\charrsid11484550 \hich\af0\dbch\af13\loch\f0 (}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \loch\af0\hich\af0\dbch\f13 \'cf\'f2\'bf\'cd\'b7\'fe\'c8\'cb\'d4\'b1\'cb\'f7\'c8\'a1\'d4\'ad\'b0\'e6}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592\charrsid11484550 \loch\af0\hich\af0\dbch\f13 \'cb\'b5\'c3\'f7\'ca\'e9 \'a3\'a9}{\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \par }\pard\plain \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 PRINCIPLE OF THE ASSAY }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\cf1\insrsid15547592 \par }\pard\plain \ltrpar\s20\qj \li0\ri0\sa427\sl551\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGB-1. St}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HMGB{\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}} {\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}-1 }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a{\*\xmlclose}-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 incubated. Then a TMB (3\hich\af1\dbch\af13\loch\f1 ,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HMGB-1, biotin-conjugated antibody }{ \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addit\hich\af1\dbch\af13\loch\f1 ion of a sulphuric acid solution }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 the color change is measured spectrophotometrically at a wavelength of 450 nm \loch\af1\dbch\af13\hich\f1 \'b1 \hich\af1\dbch\af13\loch\f1 2 nm. The concentration of HMGB{\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}-1 in{\*\xmlclose} the samples is then determined by comparing the O.D. of the samples to the st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve. \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 DETECTION R\hich\af1\dbch\af13\loch\f1 ANGE }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\cf1\insrsid15547592 \par }\pard\plain \ltrpar\s19\qj \li0\ri0\sl551\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 78 pg/ml-5000 pg/ml. The st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve concentrations used for the ELISA\hich\f1 \rquote \loch\f1 s were 5000 pg/ml, 2500 pg/ml, 1250 pg/ml, 625pg/ml, 312 pg/ml, 156 pg/ml, 78 pg/ml. \par }\pard\plain \ltrpar\s17\ql \li0\ri0\sa252\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 SPECIFICITY }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\cf1\insrsid15547592 \par }\pard\plain \ltrpar\s20\ql \li0\ri0\sa427\sl540\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 This assay recognizes human HMGB-1. No significant cross-reactivity or interf\hich\af1\dbch\af13\loch\f1 erence was observed. \par }\pard\plain \ltrpar\s17\ql \li0\ri0\sa252\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 SENSITIVITY }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\cf1\insrsid15547592 \par }\pard\plain \ltrpar\s20\ql \li0\ri0\sa427\sl540\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 The minimum detectable dose of human HMGB-1 is typically less than 20 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated fr\hich\af1\dbch\af13\loch\f1 om zero. \par }\pard\plain \ltrpar\s17\ql \li0\ri0\sa252\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\cf1\insrsid15547592 \hich\af1\dbch\af13\loch\f1 MATERIALS PROVIDED STORAGE }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\cf1\insrsid15547592 \par }\pard\plain \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1 \ltrch\fcs0 \lang1024\langfe1024\noproof\insrsid15547592 {\shp{\*\shpinst\shpleft2586\shptop8228\shpright11383\shpbottom13823\shpfhdr0\shpbxpage\shpbxignore\shpbypage\shpbyignore\shpwr5\shpwrk0\shpfblwtxt1\shpz0\shplid1026 {\sp{\sn shapeType}{\sv 202}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{\sn lTxid}{\sv 65536}}{\sp{\sn fFilled}{\sv 0}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn pWrapPolygonVertices}{\sv 8;5;(0,0);(21600,0);(21600,21600) ;(0,21600);(0,0)}}{\sp{\sn posrelh}{\sv 1}}{\sp{\sn posrelv}{\sv 1}}{\sp{\sn fLayoutInCell}{\sv 0}}{\sp{\sn fLayoutInCell}{\sv 0}}{\shptxt \ltrpar \ltrrow\trowd \irow0\irowband0\ltrrow \ts11\trgaph108\trrh155\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Reagent }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \cell }{ \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Quantity }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow0\irowband0\ltrrow\ts11\trgaph108\trrh155\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow1\irowband1\ltrrow \ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Assay plate \cell \hich\af1\dbch\af13\loch\f1 1 \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow1\irowband1\ltrrow\ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow2\irowband2\ltrrow \ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Standard \cell \hich\af1\dbch\af13\loch\f1 2 \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow2\irowband2\ltrrow\ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow3\irowband3\ltrrow \ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Sample Diluent \cell \hich\af1\dbch\af13\loch\f1 1 x 20 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow3\irowband3\ltrrow\ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow4\irowband4\ltrrow\ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody Diluent \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow4\irowband4\ltrrow \ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow5\irowband5\ltrrow \ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin Diluent \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow5\irowband5\ltrrow\ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow6\irowband6\ltrrow\ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody \cell \hich\af1\dbch\af13\loch\f1 1 x 120}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow6\irowband6\ltrrow \ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow7\irowband7\ltrrow \ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin \cell \hich\af1\dbch\af13\loch\f1 1 x 120}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow7\irowband7\ltrrow\ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow8\irowband8\ltrrow \ts11\trgaph108\trrh150\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \cell }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 1 x 20 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow8\irowband8\ltrrow\ts11\trgaph108\trrh150\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow9\irowband9\ltrrow\ts11\trgaph108\trrh100\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Wash\hich\af1\dbch\af13\loch\f1 Buffer \cell }{\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \trowd \irow9\irowband9\ltrrow \ts11\trgaph108\trrh100\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow10\irowband10\ltrrow \ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \cell }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 (25 \loch\af1\dbch\af13\hich\f1 \'d7\hich\af1\dbch\af13\loch\f1 concentrate) \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow10\irowband10\ltrrow \ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow11\irowband11\ltrrow \ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 TMB Substrate \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow11\irowband11\ltrrow\ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow12\irowband12\ltrrow\ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Stop Solution \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow12\irowband12\ltrrow \ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row }}}{\shprslt{\*\do\dobxpage\dobypage\dodhgt8192\dptxbx\dptxlrtb{\dptxbxtext\ltrpar \ltrrow\trowd \irow0\irowband0\ltrrow \ts11\trgaph108\trrh155\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Reagent }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \cell }{ \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Quantity }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow0\irowband0\ltrrow\ts11\trgaph108\trrh155\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow1\irowband1\ltrrow \ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Assay plate \cell \hich\af1\dbch\af13\loch\f1 1 \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow1\irowband1\ltrrow\ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow2\irowband2\ltrrow \ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Standard \cell \hich\af1\dbch\af13\loch\f1 2 \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow2\irowband2\ltrrow\ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow3\irowband3\ltrrow \ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Sample Diluent \cell \hich\af1\dbch\af13\loch\f1 1 x 20 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow3\irowband3\ltrrow\ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow4\irowband4\ltrrow\ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody Diluent \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow4\irowband4\ltrrow \ts11\trgaph108\trrh198\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow5\irowband5\ltrrow \ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin Diluent \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow5\irowband5\ltrrow\ts11\trgaph108\trrh187\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow6\irowband6\ltrrow\ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody \cell \hich\af1\dbch\af13\loch\f1 1 x 120}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow6\irowband6\ltrrow \ts11\trgaph108\trrh213\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow7\irowband7\ltrrow \ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin \cell \hich\af1\dbch\af13\loch\f1 1 x 120}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l \cell }\pard\plain \ltrpar \ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow7\irowband7\ltrrow\ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow8\irowband8\ltrrow \ts11\trgaph108\trrh150\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \cell }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 1 x 20 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow8\irowband8\ltrrow\ts11\trgaph108\trrh150\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow9\irowband9\ltrrow\ts11\trgaph108\trrh100\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Wash\hich\af1\dbch\af13\loch\f1 Buffer \cell }{\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \insrsid15547592 \trowd \irow9\irowband9\ltrrow \ts11\trgaph108\trrh100\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow10\irowband10\ltrrow \ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \cell }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 (25 \loch\af1\dbch\af13\hich\f1 \'d7\hich\af1\dbch\af13\loch\f1 concentrate) \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow10\irowband10\ltrrow \ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalt\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalt \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow}\trowd \irow11\irowband11\ltrrow \ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar\s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 TMB Substrate \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow11\irowband11\ltrrow\ts11\trgaph108\trrh186\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb \brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalc\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row \ltrrow }\trowd \irow12\irowband12\ltrrow\ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\pard\plain \ltrpar \s15\qc \li0\ri0\nowidctlpar\intbl\wrapdefault\faauto\rin0\lin0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Stop Solution \cell \hich\af1\dbch\af13\loch\f1 1 x 10 ml \cell }\pard\plain \ltrpar\ql \li0\ri0\widctlpar\intbl\wrapdefault\aspalpha\aspnum\faauto\adjustright\rin0\lin0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs21\lang1033\langfe2052\kerning2\loch\af0\hich\af0\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \trowd \irow12\irowband12\ltrrow \ts11\trgaph108\trrh165\trleft-108\trftsWidth1\trpaddl108\trpaddr108\trpaddfl3\trpaddfr3\tblind0\tblindtype3 \clvertalb\clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth4632\clshdrawnil \cellx4524\clvertalb \clbrdrt\brdrtbl \clbrdrl\brdrtbl \clbrdrb\brdrtbl \clbrdrr\brdrtbl \cltxlrtb\clftsWidth3\clwWidth3365\clshdrawnil \cellx7889\row }}\dpx2586\dpy8228\dpxsize8797\dpysize5595 \dpfillfgcr255\dpfillfgcg255\dpfillfgcb255\dpfillbgcr255\dpfillbgcg255\dpfillbgcb255\dpfillpat0\dplinehollow}}}}{\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \par }\pard \ltrpar\s15\ql \li0\ri0\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af0 \ltrch\fcs0 \cf0\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 1.\tab}}\pard \ltrpar\s15\ql \fi-360\li360\ri0\sa227\nowidctlpar\wrapdefault\faauto\ls2\rin0\lin360\itap0 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Unopened test kits should be stored at 2-8}{\rtlch\fcs1 \af13\afs28 \ltrch\fcs0 \f13\fs28\cf0\insrsid15547592 \loch\af13\hich\af13\dbch\f13 \uc1\u-3920\'3f}{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 C upon receipt }{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration \hich\af1\dbch\af13\loch\f1 date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 2.\tab}\hich\af1\dbch\af13\loch\f1 Opened test plate should be stored at 2-8}{\rtlch\fcs1 \af13\afs28 \ltrch\fcs0 \f13\fs28\cf0\insrsid15547592 \loch\af13\hich\af13\dbch\f13 \uc1\u-3920\'3f}{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain sta \hich\af1\dbch\af13\loch\f1 ble until the expiring date shown, provided it is stored as prescribed above. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 3.\tab}}\pard \ltrpar\s15\ql \fi-360\li360\ri0\nowidctlpar\wrapdefault\faauto\ls2\rin0\lin360\itap0 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 A microtiter plate reader with a b}{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 width of 10 nm or less }{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n\hich\af1\dbch\af13\loch\f1 -d}{\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement\hich\af1\dbch\af13\loch\f1 . \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s22\qj \li0\ri0\sa342\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 REAGENT PREPARATION }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par }\pard\plain \ltrpar\s23\qj \li0\ri0\sa122\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Bring all reagents to room temperature before use. }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\f1\fs28 \hich\af1\dbch\af13\loch\f1 1\tab}}\pard\plain \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls3\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Wash Buffer }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 If crystals have formed in the concentrate, warm up to room temperature }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concen\hich\af1\dbch\af13\loch\f1 trate into deionized or distilled water to prepare 500 ml of Wash Buffer. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\f1\fs28 \hich\af1\dbch\af13\loch\f1 2\tab}}{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 St}{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Centrifuge the st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard vial at 6000-10000rpm for 30s. Reconstitute the }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 St}{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{ \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 with 1.0 ml of }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Sample Diluent}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 . This reconstitution produces a stock solution of 5000pg/ml. \hich\af1\dbch\af13\loch\f1 Allow the st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard serves as the high st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard (5000pg/ml). The }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Sample Diluent }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 serves as the zero st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard (0 pg/ml). Prepare fresh for each a \hich\af1\dbch\af13\loch\f1 ssay. Use within 4 hours }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 discard after use. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\f1\fs28 \hich\af1\dbch\af13\loch\f1 3\tab}}{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Centrifuge the vial before opening. Dilute to the working concentration using }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody Diluent}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 (1:100), respectively. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\f1\fs28 \hich\af1\dbch\af13\loch\f1 4\tab}}{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Centrifuge the vial before opening. Dilute to the worki\hich\af1\dbch\af13\loch\f1 ng concentration using }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin Diluent}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 (1:100), respectively. \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s20\qj \fi-1105\li1105\ri0\sa427\sl398\slmult0\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin1105\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, h}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 , face, }{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 clothing protection when using this material. }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 OTHER SUPPLIES REQUIRED }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 1\tab}}\pard\plain \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls4\rin0\lin0\itap0\pararsid1590987 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Microplate reader \hich\af1\dbch\af13\loch\f1 capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 2\tab}}\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls4\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Pipettes }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 pipette tips. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 3\tab}\hich\af1\dbch\af13\loch\f1 Deionized or distilled water. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 4\tab}\hich\af1\dbch\af13\loch\f1 Squirt bottle, manifold dispenser, or automated microplate washer. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 5\tab}\hich\af1\dbch\af13\loch\f1 An incubator which can provid\hich\af1\dbch\af13\loch\f1 e stable incubation conditions up to {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}} {\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}37\loch\af1\dbch\af13\hich\f1 \'b0 \hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}\loch\af1\dbch\af13\hich\f1 \'b1{\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}} {\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}\hich\af1\dbch\af13\loch\f1 0.5\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}. \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 SAMPLE COLLECTION }{\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid12795595 \hich\af1\dbch\af13\loch\f1 A-N-D}{\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 STORAGE }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par }\pard\plain \ltrpar\s15\ql \fi701\li0\ri0\sa285\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0\cufi250\pararsid1590987 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 { \rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Serum }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Use a serum separator tube (SST) }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 allow samples to clot for 30 minutes before centrifugation for 15 minutes at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}} {\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}1000 g{\*\xmlclose}. Remove serum }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 assay immediately or aliquot }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 store samples at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC} {\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}-20\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose} . Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles. \par }\pard \ltrpar\s15\ql \fi701\li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0\cufi250\pararsid1590987 {\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Plasma }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}} {\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}1000 g{\*\xmlclose} within 30 minutes of \hich\af1\dbch\af13\loch\f1 collection. Assay immediately or aliquot }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 store samples at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}} {\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}-20\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles. \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s18\qj \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Note: Grossly hemolyzed samples are not suitable for use in this assay. }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard\plain \ltrpar\s22\qj \li0\ri0\sa342\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ASSAY PROCEDURE }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\sl400\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Bring all reagents }{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 samples to room temperature before use. It is recommended that all samples, st}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ards, }{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 controls be assayed in duplicate. }{\field{\*\fldinst {\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HYPERLINK "http://www.iciba.com/liquid/"}{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592\charrsid13697845 {\*\datafield 00d0c9ea79f9bace118c8200aa004ba90b0200000003000000e0c9ea79f9bace118c8200aa004ba90b5200000068007400740070003a002f002f007700770077002e00690063006900620061002e0063006f006d002f006c00690071007500690064002f000000795881f43b1d7f48af2c825dc485276300000000a5ab0000} }}{\fldrslt {\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 All the reagents should be added directly to the liquid level in the\hich\af1\dbch\af13\loch\f1 well. }}}\sectd \linex0\sectdefaultcl\sftnbj { \rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 The pipette should avoid contacting the inner wall of the well. }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 1\tab}}\pard\plain \ltrpar\s15\ql \li0\ri0\sa335\nowidctlpar\wrapdefault\faauto\ls6\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Add 100}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\cf0\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l of St}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}} {\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}37\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 2\tab}\hich\af1\dbch\af13\loch\f1 Remove the liquid of each well, don\hich\f1 \rquote \loch\f1 t wash. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 3\tab}\hich\af1\dbch\af13\loch\f1 Add 100}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\cf0\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{ \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l of }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-anti\hich\af1\dbch\af13\loch\f1 body }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 working solution to each well. Incubate for 1 hour at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}} {\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}37\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}. }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Biotin-antibody }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 working solution may appear cloudy. Warm up to room temperature }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 mix gently until solution appears uniform. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 4\tab}}\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls6\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Aspirate each well }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 wash, repeating the process three times \hich\af1\dbch\af13\loch\f1 for a total of three washes. Wash: Fill each well with \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s25\qj \li420\ri0\sl631\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin420\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Wash Buffer (200}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l) }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 let it st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 for 2 minutes, then \par \hich\af1\dbch\af13\loch\f1 remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal \hich\af1\dbch\af13\loch\f1 of liquid at each step is essential to good performance. \par }\pard\plain \ltrpar\s15\ql \li0\ri0\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 1\tab}}\pard \ltrpar\s15\ql \li0\ri0\sa268\nowidctlpar\wrapdefault\faauto\ls7\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Add 100}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\cf0\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l of }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 HRP-avidin }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType} {\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}}{\xmlattr\xmlattrns0{\xmlattrname UnitName }{\xmlattrvalue \'a1\'e3C}}}37\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 2\tab}\hich\af1\dbch\af13\loch\f1 Repeat the aspiration }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 wash five times as step 4. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 3\tab}\hich\af1\dbch\af13\loch\f1 Add \hich\af1\dbch\af13\loch\f1 90}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\cf0\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l of }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 TMB Substrate }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 to each well. Incubate for 10-30 minutes at {\*\xmlopen\xmlns2{\factoidname chmetcnv}{\xmlattr\xmlattrns0{\xmlattrname TCSC}{\xmlattrvalue 0}}{\xmlattr\xmlattrns0{\xmlattrname NumberType}{\xmlattrvalue 1}}{\xmlattr\xmlattrns0{\xmlattrname Negative}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname HasSpace}{\xmlattrvalue False}}{\xmlattr\xmlattrns0{\xmlattrname SourceValue}{\xmlattrvalue 37}} {\xmlattr\xmlattrns0{\xmlattrname UnitName}{\xmlattrvalue \'a1\'e3C}}}37\loch\af1\dbch\af13\hich\f1 \'b0\hich\af1\dbch\af13\loch\f1 C{\*\xmlclose}. Keeping the plate away from drafts }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 other temperature fluctuations in the dark. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 4\tab}\hich\af1\dbch\af13\loch\f1 Add 50}{\rtlch\fcs1 \af52\afs28 \ltrch\fcs0 \f52\fs28\cf0\insrsid15547592 \loch\af52\dbch\af13\hich\f52 \'ec}{ \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 l of }{\rtlch\fcs1 \ab\af1\afs28 \ltrch\fcs0 \b\fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Stop Solution }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 to each well when the first four wells containing the highest concentratio\hich\af1\dbch\af13\loch\f1 n of st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \f1\fs28 \hich\af1\dbch\af13\loch\f1 5\tab}}\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls7\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm. \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s22\qj \li0\ri0\sa342\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 CALCULATION OF RES\hich\af1\dbch\af13\loch\f1 ULTS }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\sl400\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Using the professional soft "Curve Exert 1.3" to make a st}{\rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{ \rtlch\fcs1 \ab\ai\af1\afs21 \ltrch\fcs0 \b\i\fs21\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve is recommended, which can be downloaded from our web. }{\rtlch\fcs1 \af1\afs21 \ltrch\fcs0 \fs21\insrsid15547592 \par }\pard\plain \ltrpar\s20\qj \li0\ri0\sa427\sl551\slmult0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Average the duplicate readings for each st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard, control, }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 sample }{ \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 subtract the average zero st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-\hich\af1\dbch\af13\loch\f1 d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard optical density\hich\af1\dbch\af13\loch\f1 . Create a st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{ \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve by plotting the mean absorbance for each st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard on the x-axis against t\hich\af1\dbch\af13\loch\f1 he concentration on the y-axis }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HMGB-1 concentrations versus the log of the O.D. }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 the best fit line can be determined by regression analysis.\hich\af1\dbch\af13\loch\f1 This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve must be multiplied by the dilution factor. \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 LIMITATIONS OF THE PROCEDURE }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 1\tab}}\pard\plain \ltrpar\s15\ql \li0\ri0\sa256\nowidctlpar\wrapdefault\faauto\ls8\rin0\lin0\itap0\pararsid4222265 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 The kit should not be used bey \hich\af1\dbch\af13\loch\f1 ond the expiration date on the kit label. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 2\tab}}\pard \ltrpar\s15\ql \li0\ri0\sa256\nowidctlpar\wrapdefault\faauto\ls8\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Do not mix or substitute reagents with those from other lots or sources. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 3\tab}\hich\af1\dbch\af13\loch\f1 It is important that the St}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard Diluent selected for the st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{ \rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard curve be consistent with the samples being assayed. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 4\tab}\hich\af1\dbch\af13\loch\f1 If samples generat\hich\af1\dbch\af13\loch\f1 e values higher than the highest st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard, dilute the samples with the appropriate St}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard Diluent }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 repeat the assay. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 5\tab}\hich\af1\dbch\af13\loch\f1 Any variation in St}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 kit age can \hich\af1\dbch\af13\loch\f1 cause variation in binding. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 6\tab}}\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls8\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 This assay is designed to eliminate interference by soluble receptors, binding proteins, }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference ca\hich\af1\dbch\af13\loch\f1 nnot be excluded. \par }\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \par }\pard\plain \ltrpar\s17\qj \li0\ri0\sa252\pagebb\nowidctlpar\wrapdefault\faauto\rin0\lin0\itap0 \rtlch\fcs1 \af0\afs24\alang1025 \ltrch\fcs0 \fs24\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \ab\af1\afs32 \ltrch\fcs0 \b\fs32\insrsid15547592 \hich\af1\dbch\af13\loch\f1 TECHNICAL HINTS }{\rtlch\fcs1 \af1\afs32 \ltrch\fcs0 \fs32\insrsid15547592 \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 1\tab}}\pard\plain \ltrpar\s15\ql \li0\ri0\sa254\nowidctlpar\wrapdefault\faauto\ls9\rin0\lin0\itap0\pararsid4222265 \rtlch\fcs1 \af1\afs24\alang1025 \ltrch\fcs0 \fs24\cf1\lang1033\langfe2052\loch\af1\hich\af1\dbch\af13\cgrid\langnp1033\langfenp2052 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Centrifuge vials before opening to collect contents. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 2\tab}}\pard \ltrpar\s15\ql \li0\ri0\sa254\nowidctlpar\wrapdefault\faauto\ls9\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 When mixing or reconstituting protein solutions, always avoid foaming. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 3\tab}\hich\af1\dbch\af13\loch\f1 To avoid cross-contamination, change pipette tips between additions of each st}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 ard level, b\hich\af1\dbch\af13\loch\f1 etween sample additions, }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 between reagent additions. Also, use separate reservoirs for each reagent. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 4\tab}\hich\af1\dbch\af13\loch\f1 When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, }{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid12795595 \hich\af1\dbch\af13\loch\f1 a-n-d}{\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 /or rotating the plate 180 degrees betwe\hich\af1\dbch\af13\loch\f1 en wash steps may improve assay precision. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 5\tab}\hich\af1\dbch\af13\loch\f1 To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 6\tab}\hich\af1\dbch\af13\loch\f1 Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution prote\hich\af1\dbch\af13\loch\f1 cted from light. Substrate Solution should change from colorless or light blue to gradations of blue. \par {\listtext\pard\plain\ltrpar \s15 \rtlch\fcs1 \af0\afs28 \ltrch\fcs0 \fs28\loch\af0\dbch\af0 \hich\af0\dbch\af0\loch\f0 7\tab}}\pard \ltrpar\s15\ql \li0\ri0\nowidctlpar\wrapdefault\faauto\ls9\rin0\lin0\itap0 {\rtlch\fcs1 \af1\afs28 \ltrch\fcs0 \fs28\cf0\insrsid15547592 \hich\af1\dbch\af13\loch\f1 Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow up\hich\af1\dbch\af13\loch\f1 on addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution. \par }}